Application

Anti-Myomesin-2, clone 3B9.3, Cat. No. MABT94, is a mouse monoclonal antibody that detects human Myomesin-2 and has been tested for use in Western Blotting.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myomesin-2 (UniProt: P54296; also known as 165 kDa connectin-associated protein, 165 kDa titin-associated protein, M-protein, Myomesin family member 2) is encoded by the MYOM2 gene (Gene ID: 9172) in human. Myomesin-2 is a major component of the vertebrate myofibrillar M band and is mainly expressed in fast fibers. It binds myosin, titin, and light meromyosin in a dose-dependent manner. Its primary function is to upkeep M-band filament lattice. It has a unique N-terminal domain followed by 12 repeat domains with strong homology to either fibronectin type III or immunoglobulin C2 domains. Myomesin-2 is shown to interact with dysferlin, a protein that aids in repairing the sarcolemma when it is damaged or torn due to muscle strain and assists in myofibril stability.

Immunogen

GST-tagged recombinant fragment corresponding to 154 amino acids from the N-terminal region of human Myomesin-2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Myomesin-2 in human skeletal muscle tissue lysate.

Specificity

Clone 3B9.3 detects human muscle Myomesin-2. It targets an epitope with in the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~165 kDa observed; 164.90 kDa calculated. Uncharacterized bands may be observed in some lysate(s).